This fixture looks to be a formidable task for Wigan as City are chasing the quadruple.
Paul Cook will have to look at clips from City’s only two defeats this season, against Liverpool and Shakhtar Donetsk to try to exploit the weaknesses of Guardiola’s team.
City will be confident as trio Leroy Sane, David Silva and Fabian Delph all returned from injury to play in the 4-0 Champions League victory at Basel last week.
The Latics will be missing captain, Sam Morsy, who begins a two-match suspension after being shown his 10th yellow card of the season in last week’s defeat by Blackpool.
Wigan manager Paul Cook said: “We’ve got a fantastic stadium and the support inside it will make it a really good atmosphere, that’s a guarantee. Everyone can look forward to a good night of football.”
This is the fifth meeting between these two sides in the FA Cup, with Wigan winning the last two: the 2013 FA Cup final with Ben Watson grabbing the winner and a quarter-final away tie the following season.